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Development of a Real-Time PCR Assay for Detection of<i>Toxoplasma gondii</i>in Pig and Mouse Tissues

1 Jun 2001

Abstract excerpt

A highly sensitive and specific method has been developed to reproducibly detect and quantitate Toxoplasma gondii burden in animal tissue samples using T. gondii ITS1-derived primers and a fluorogenic probe via real-time PCR. Assay specificity was confirmed against a panel of DNA samples from T. gondii and other common protozoa as well as host animal tissue. This Toxo TaqMan assay was able to detect as little as...

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PMID: 11376036
DOI: 10.1128/jcm.39.6.2065-2071.2001

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