Article
[Simultaneous detection and differentiation of O1 classical E1 Tor, O139 and nonO1 nonO139 strains of Vibrio cholerae by using multiplex PCR].
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] - 1 Sept 2000
Rui Y, Cai C, Xiao B
Abstract excerpt
OBJECTIVE: To explore the rapid and sensitive procedures for detection and differentiation of O1 classical(CVC), El Tor(EVC), O139 and nonO1 nonO139 strains of Vibrio cholerae. METHODS: Four pairs of primers were designed from toxin sub-unit A(ctxA) gene, O139 special gene, Haemolytic subunit A gene and Toxin coregulated pilus subunit A gene. Multiplex PCR was established to simultaneously detect those four...
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