Article
[Analysis of characteristics of major pathogenicity-related genes of Vibrio cholerae isolated in Guangzhou area from 2001 to 2005].
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] - 1 Jul 2006
Wang Ming, Li Xiao-quan, Mo Zi-yao, Liu Yu-fei, Deng Zhi-ai, Shen Ji-chuan, Zhang Xin-qiang
Abstract excerpt
OBJECTIVE: To apply multiplex polymerase chain reaction (MPCR) assay and sequencing in study of the carrying status of four pathogenicity-related genes of Vibrio cholerae (V.cholerae) and the variation of ctxA. METHODS: Primers targeting cholera toxin sub-unit A gene (ctxA), toxin-coregulated pilus gene (tcpA), accessory cholera enterotoxin gene (ace), zonula occludens toxin gene (zot) were designed and the MPCR...
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