Article
Use of fluorescent Ca2+ dyes with green fluorescent protein and its variants: problems and solutions.
The Biochemical journal - 1 Jun 2001
Bolsover S, Ibrahim O, O'luanaigh N, Williams H, Cockcroft S
Abstract excerpt
We have studied the degree to which fluorescent Ca(2+) indicator dyes, and green fluorescent protein and its variants, can be used together. We find that the most commonly used fluorescent protein, enhanced green fluorescent protein (EGFP), seriously contaminates fura 2 signals. We suggest two alternative combinations for which there is no detectable contamination of the Ca(2+) indicator signal by the fluorescent...
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