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Allele-tagged TaqMan® PCR genotyping assays for high-throughput detection of soybean cyst nematode resistance.

Molecular biology reports - 2 Dec 2024

Usovsky Mariola, Bilyeu Kristin, Bent Andrew, Scaboo Andrew M

Abstract excerpt

BACKGROUND: Whole genome resequencing (WGRS) platforms provide exceptional fingerprinting of the entire genome but are expensive and less flexible to use as a routine genotyping tool for targeting causal polymorphisms within a germplasm collection or breeding program. Therefore, there has been a continuous effort to develop small-scale genotyping platforms that facilitate robust and quick assessments of the...

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PMID: 39621159
DOI: 10.1007/s11033-024-10114-6
PMCID: PMC11611941

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