Article
Detection of double-stranded PCR amplicons at the attomole level electrosprayed from low nanomolar solutions using FT-ICR mass spectrometry.
Fresenius' journal of analytical chemistry - 1 Feb 2001
Hannis J C, Muddiman D C
Abstract excerpt
An 82-base-pair polymerase chain reaction (PCR) product was amplified from the tetranucleotide short tandem repeat locus within the human tyrosine hydroxylase gene. PCR amplification was carried out using 100 ng of human nuclear DNA obtained from an individual who is homozygotic for the 9.3 allele resulting in a 50.5 kDa amplicon. To generate sufficient material for these investigations, several reactions were...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.