Article
Optimizing the detection of nascent transcripts by RNA fluorescence in situ hybridization.
Nucleic acids research - 15 Apr 2001
van Raamsdonk C D, Tilghman S M
Abstract excerpt
An unusual feature of the mammalian genome is the number of genes exhibiting monoallelic expression. Recently random monoallelic expression of autosomal genes has been reported for olfactory and Ly-49 NK receptor genes, as well as for Il-2, Il-4 and Pax5. RNA fluorescence in situ hybridization (FISH) has been exploited to monitor allelic expression by visualizing the number of sites of transcription in individual...
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