Article
Cold-temperature plastic resin embedding of liver for DNA- and RNA-based genotyping.
The Journal of molecular diagnostics : JMD - 1 Nov 1999
Finkelstein S D, Dhir R, Rabinovitz M, Bischeglia M, Swalsky P A, DeFlavia P, Woods J, Bakker A, Becich M
Abstract excerpt
The standard practice of tissue fixation in 10% formalin followed by embedding in paraffin wax preserves cellular morphology at the expense of availability and quality of DNA and RNA. The negative effect on cellular constituents results from a combination of extensive cross-linking and strand scission of DNA, RNA, and proteins induced by formaldehyde as well as RNA loss secondary to ubiquitous RNase activity and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.