Article
Detection of avocado sunblotch viroid variants using fluorescent single-strand conformation polymorphism analysis.
Electrophoresis - 1 Feb 2001
Schnell R J, Olano C T, Kuhn D N
Abstract excerpt
A specific reverse transcription-polymerase chain reaction (RT-PCR) protocol has been developed for routine detection of avocado sunblotch viroid (ASBVd). Modifications in this diagnostic technique were made to enable fluorescent detection and variant identification using automated capillary electrophoresis (CE) and fluorescent single-strand conformation polymorphism (SSCP) analysis. Sixteen sequence variants...
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