Article
Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid gene.
Archives of virology - 1 Jan 2009
Kubo Karen Sumire, Stuart R M, Freitas-Astúa J, Antonioli-Luizon R, Locali-Fabris E C, Coletta-Filho H D, Machado M A, Kitajima E W
Abstract excerpt
The variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of 18 genera of orchids were collected from Brazil, Costa Rica and Australia. The SSCP analysis yielded six different band patterns, and phylogenetic analysis based on the nucleotide fragment sequence...
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