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Article

Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis.

Journal of clinical microbiology - 1 Feb 2001

Allard A, Albinsson B, Wadell G

Abstract excerpt

We have developed a system for rapid typing of adenoviruses (Ads) based on a combination of PCR and restriction endonuclease (RE) digestion (PCR-RE digestion). Degenerated consensus primers were designed, allowing amplification of DNA from all 51 human Ad prototype strains and altogether 44 different genome variants of Ad serotypes 1, 3, 4, 5, 7, 11, 19, 40, and 41. The 301-bp amplimer of 22 prototype strains...

Topics

Adenoviridae Infections
Adenoviruses, Human
Adult
Aged
Cell Line
Child, Preschool
DNA Primers
Genetic Variation
Genome, Viral
Humans
Infant
Middle Aged

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phylogeny

Polymerase Chain Reaction

Restriction Mapping

Serotyping

Source

Open in PubMed Open full text

Identifiers

PMID: 11158096
DOI: 10.1128/JCM.39.2.498-505.2001
PMCID: PMC87765

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