Beta-ketothiolase genes in Azotobacter vinelandii.

doi:10.1016/s0378-1119(00)00462-5

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Article

Beta-ketothiolase genes in Azotobacter vinelandii.

Gene - 30 Dec 2000

Segura D, Vargas E, Espín G

Abstract excerpt

Azotobacter vinelandii is proposed to contain a single beta-ketothiolase activity participating in the formation of acetoacetyl-CoA, a precursor for poly-beta-hydroxybutyrate (PHB) synthesis, and in beta-oxidation (Manchak, J., Page, W.J., 1994. Control of polyhydroxyalkanoate synthesis in Azotobacter vinelandii strain UWD. Microbiology 140, 953-963). We designed a degenerate oligonucleotide from a highly...

Topics

3-Hydroxyacyl CoA Dehydrogenases
Acetyl-CoA C-Acyltransferase
Amino Acid Sequence
Azotobacter vinelandii
Cell Division
Cloning, Molecular
DNA, Bacterial
Fatty Acids
Genes, Bacterial
Genotype
Hydroxybutyrates
Molecular Sequence Data
Mutation
Oxidation-Reduction
Polyesters
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid

Source

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Identifiers

PMID: 11137297
DOI: 10.1016/s0378-1119(00)00462-5

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