Transmembrane electron transfer by the membrane protein DsbD occurs via a disulfide bond cascade.

doi:10.1016/s0092-8674(00)00180-x

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Article

Transmembrane electron transfer by the membrane protein DsbD occurs via a disulfide bond cascade.

Cell - 22 Nov 2000

Katzen F, Beckwith J

Abstract excerpt

The cytoplasmic membrane protein DsbD transfers electrons from the cytoplasm to the periplasm of E. coli, where its reducing power is used to maintain cysteines in certain proteins in the reduced state. We split DsbD into three structural domains, each containing two essential cysteines. Remarkably, when coexpressed, these truncated proteins restore DsbD function. Utilizing this three piece system, we were able...

Topics

Blotting, Western
Cysteine
Cytoplasm
Disulfides
Electron Transport
Escherichia coli
Genotype
Models, Biological
Oxidation-Reduction
Periplasm
Phenotype
Plasmids
Protein Disulfide-Isomerases
Protein Structure, Tertiary
Thioredoxins

Source

Open in PubMed

Identifiers

PMID: 11114333
DOI: 10.1016/s0092-8674(00)00180-x

Provenance

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