Article
Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction.
Molecular and biochemical parasitology - 1 Nov 2000
Menegon M, Severini C, Sannella A, Paglia M G, Sangaré D, Abdel-Wahab A, Abdel-Muhsin A A, Babiker H, Walliker D, Alano P
Abstract excerpt
A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript abundantly produced in maturing gametocytes. The gene contains four...
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