Article
Characterization of an OprF-deficient mutant suggests that OprF is an essential protein for Pseudomonas fluorescens strain MF0.
Research in microbiology - 1 Oct 2000
Chevalier S, Burini J F, Freulet-Marriere M A, Regeard C, Schoofs G, Guespin-Michel J, De Mot R, Orange N
Abstract excerpt
A stable OprF-deficient mutant for Pseudomonas fluorescens strain MF0 was constructed using reverse genetics. This mutant, called MF372, showed a rounded morphology and grew more slowly in minimal medium, but not in rich medium. Contrary to other Pseudomonas strains, the loss of OprF for strain MF0 was accompanied by an altered outer membrane composition. At least three outer membrane proteins were overexpressed,...
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