Cytochrome c550 is an essential component of the quinoprotein ethanol oxidation system in Pseudomonas aeruginosa: cloning and sequencing of the genes encoding cytochrome c550 and an adjacent acetaldehyde dehydrogenase.

Abstract excerpt

Pseudomonas aeruginosa ATCC 17933 grown aerobically on ethanol produces a soluble cytochrome c550 together with a quinoprotein ethanol dehydrogenase. A 3.2 kb genomic DNA fragment containing the gene encoding cytochrome c550 was cloned and sequenced. Two other complete and two truncated ORFs were also identified. A truncated ORF encoding the quinoprotein ethanol dehydrogenase (exaA) was found upstream of the...

Topics

• Aldehyde Oxidoreductases
• Amino Acid Sequence
• Cloning, Molecular
• Cytochrome c Group
• Escherichia coli
• Ethanol
• Genes, Bacterial
• Genetic Complementation Test
• Molecular Sequence Data
• Mutation
• Oxidation-Reduction
• Physical Chromosome Mapping