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Article

Flow cytometry characterisation of Salmonella typhimurium mutants defective in proton translocating proteins and stationary-phase growth phenotype.

Journal of microbiological methods - 1 Nov 2000

Rychlik I, Cardova L, Sevcik M, Barrow P A

Abstract excerpt

We have shown that the growth, starvation and population heterogeneity of Salmonella typhimurium and its isogenic nuoG and cydA mutants can be monitored by flow cytometry. Bacterial cells were analysed unstained, and after staining with rhodamine 123, propidium iodide and acridine orange. In unstained cultures it was possible to distinguish flagellated and non-flagellated cells. nuoG and cydA mutants were less...

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PMID: 11044569
DOI: 10.1016/s0167-7012(00)00199-8

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