Article
Immortalization of bovine dental papilla cells with simian virus 40 large t antigen.
Archives of oral biology - 1 Oct 2000
Thonemann B, Schmalz G
Abstract excerpt
Primary cultures of dental papilla-derived cells have a limited lifespan in vitro and can be maintained only up to passage 7-9 before showing senescence, but in vitro investigations often require a large number of cells showing phenotypic characteristics of the original tissue. To overcome this shortcoming, second-passage cells established from calf molar tooth germs by enzymatic pretreatment of the dental...
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