Article
Reduction of Cob(III)alamin to Cob(II)alamin in Salmonella enterica serovar typhimurium LT2.
Journal of bacteriology - 1 Aug 2000
Fonseca M V, Escalante-Semerena J C
Abstract excerpt
Reduction of the cobalt ion of cobalamin from the Co(III) to the Co(I) oxidation state is essential for the synthesis of adenosylcobalamin, the coenzymic form of this cofactor. A cob(II)alamin reductase activity in Salmonella enterica serovar Typhimurium LT2 was isolated to homogeneity. N-terminal analysis of the homogeneous protein identified NAD(P)H:flavin oxidoreductase (Fre) (EC 1.6.8.1) as the enzyme...
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