The dual-specificity protein phosphatase Yvh1p regulates sporulation, growth, and glycogen accumulation independently of catalytic activity in Saccharomyces cerevisiae via the cyclic AMP-dependent protein kinase cascade.

Abstract excerpt

Yvh1p, a dual-specific protein phosphatase induced specifically by nitrogen starvation, regulates cell growth as well as initiation and completion of sporulation. We demonstrate that yvh1 disruption mutants are also unable to accumulate glycogen in stationary phase. A catalytically inactive variant of yvh1 (C117S) and a DNA fragment encoding only the Yvh1p C-terminal 159 amino acids (which completely lacks the...

Topics

• Cyclic AMP
• Cyclic AMP-Dependent Protein Kinases
• Dual-Specificity Phosphatases
• Gene Deletion
• Gene Expression Regulation, Fungal
• Genes, Reporter
• Genetic Complementation Test
• Glycogen
• Mutation
• Protein Tyrosine Phosphatases
• Saccharomyces cerevisiae
• Saccharomyces cerevisiae Proteins