Article
Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway.
Molecular microbiology - 1 Jun 2000
Dassler T, Maier T, Winterhalter C, Böck A
Abstract excerpt
A chromosomal fragment has been identified in a gene bank from Escherichia coli, which augmented the yield of cysteine in an industrial production strain. Subcloning and genetic analysis showed that an open reading frame coding for a product of 299 amino acids (Orf299) was responsible. Orf299 was synthesized in the T7 polymerase/promoter system and exhibited the properties of an integral membrane protein....
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