Article
Identification of the Hind III polymorphic site in the PAI-1 gene: analysis of the PAI-1 Hind III polymorphism by PCR.
Genetic testing - 1 Jan 2000
Grenett H E, Khan N, Jiang W, Booyse F M
Abstract excerpt
The identification of the Hind III polymorphic site in the 3' end of the plasminogen activator inhibitor 1 (PAI-1) gene and a simple method to identify the Hind III polymorphism rapidly in the PAI-1 gene using PCR is described. The Hind III restriction site was identified by restriction site mapping and sequence analysis from a cosmid DNA clone. Genomic DNA was isolated from individual human umbilical cords and a...
Topics
- 3' Untranslated Regions
- Base Sequence
- Blotting, Southern
- DNA
- Deoxyribonuclease HindIII
- Electrophoresis, Agar Gel
- Evaluation Studies as Topic
- Fetal Blood
- Genotype
- Humans
- Molecular Sequence Data
- Plasminogen Activator Inhibitor 1
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Reproducibility of Results
- Sequence Analysis, DNA
- Time Factors