Article
High-resolution NMR spectroscopy of lipid A molecules containing 4-amino-4-deoxy-L-arabinose and phosphoethanolamine substituents. Different attachment sites on lipid A molecules from NH4VO3-treated Escherichia coli versus kdsA mutants of Salmonella typhimurium.
The Journal of biological chemistry - 5 May 2000
Zhou Z, Ribeiro A A, Raetz C R
Abstract excerpt
When Escherichia coli are grown on LB broth containing 25 mm NH(4)VO(3), complex modifications to the lipid A anchor of lipopolysaccharide are induced. Six modified lipid As (EV1-EV6) have been purified. Many of these variants possess 4-amino-4-deoxy-l-arabinose (l-Ara4N) and/or phosphoethanolamine (pEtN) substituents. Here we use NMR spectroscopy to investigate the attachment sites of the l-Ara4N and pEtN...
Topics
- Aldehyde-Lyases
- Amino Sugars
- Escherichia coli
- Ethanolamines
- Lipid A
- Magnetic Resonance Spectroscopy
- Mutation
- Salmonella typhimurium