Article
Nuclear localization of the human mutY homologue hMYH.
Journal of cellular biochemistry - 1 Apr 2000
Tsai-Wu J J, Su H T, Wu Y L, Hsu S M, Wu C H
Abstract excerpt
The cDNA of the human mutY homologue (hMYH) was cloned from the total RNA of the tumor cell line SU-DHL-1 by reverse transcription-polymerase chain reaction (RT-PCR). Expression of hMYH in a plasmid can partially revert the mutator phenotype of the Escherichia coli mutY mutant MK609(DE3). The majority of the recombinant hMYH protein in E. coli was precipitated in the inclusion bodies. A minor fraction of the...
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