Article
High-performance thin-layer chromatography method for inositol phosphate analysis.
Journal of chromatography. B, Biomedical sciences and applications - 24 Dec 1999
Hatzack F, Rasmussen S K
Abstract excerpt
A simple and inexpensive high-performance thin-layer chromatography (HPTLC) method for the analysis of inositol mono- to hexakisphosphates on cellulose precoated plates is described. Plates were developed in 1-propanol-25% ammonia solution-water (5:4:1) and substance quantities as low as 100-200 pmol were detected by molybdate staining. Chromatographic mobilities of nucleotides and phosphorylated carbohydrates...
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