Article
Tagging potato leafroll virus with the jellyfish green fluorescent protein gene.
The Journal of general virology - 1 Mar 2000
Nurkiyanova K M, Ryabov E V, Commandeur U, Duncan G H, Canto T, Gray S M, Mayo M A, Taliansky M E
Abstract excerpt
A full-length cDNA corresponding to the RNA genome of Potato leafroll virus (PLRV) was modified by inserting cDNA that encoded the jellyfish green fluorescent protein (GFP) into the P5 gene near its 3' end. Nicotiana benthamiana protoplasts electroporated with plasmid DNA containing this cDNA behind the 35S RNA promoter of Cauliflower mosaic virus became infected with the recombinant virus (PLRV-GFP). Up to 5% of...
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