Article
Improved detection of human immunodeficiency virus type 1 variants by analysis of replicate amplification reactions: relevance to studies of human immunodeficiency virus type 1 vertical transmission.
Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology - 1 Dec 1999
Becker-Pergola G, Mellquist J L, Eshleman J R, Jackson J B, Eshleman S H
Abstract excerpt
BACKGROUND: Human immunodeficiency virus type 1 (HIV-1)-infected individuals typically harbor mixtures of HIV-1 variants. For HIV-1 transmission studies, methods used for genotypic analysis should reliably detect variant mixtures. Such studies typically analyze complementary DNAs (cDNAs) from a single polymerase chain reaction (PCR) amplification. This approach may fail to detect variant mixtures in some samples...
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