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Six cloned calves produced from adult fibroblast cells after long-term culture

1 Feb 2000

Abstract excerpt

Cloning whole animals with somatic cells as parents offers the possibility of targeted genetic manipulations in vitro such as "gene knock-out" by homologous recombination. However, such manipulation requires prolonged culture of nuclear donor cells. Previous successes in cloning have been limited to the use of cells collected either fresh or after short-term culture. Therefore, demonstration of genetic...

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PMID: 10655472
DOI: 10.1073/pnas.97.3.990

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