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Article

L-type voltage-dependent calcium channel alpha-1C subunit mRNA is present in ejaculated human spermatozoa

1 Feb 2000

Abstract excerpt

The current study adds to the growing body of evidence that RNA is present in mature ejaculated human spermatozoa. We report that a sodium dodecyl sulphate (SDS)/citric acid extraction method is superior to guanidinium isothiocyanate in terms of reproducibility of RNA recovery from motile sperm populations from individual ejaculates. Using the SDS/citric acid method, RNA was recovered from both fresh and...

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PMID: 10655454
DOI: 10.1093/molehr/6.2.127

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