Article
Transposon tagging of the sulfur gene of tobacco using engineered maize Ac/Ds elements.
Genetics - 1 Dec 1999
Fitzmaurice W P, Nguyen L V, Wernsman E A, Thompson W F, Conkling M A
Abstract excerpt
The Sulfur gene of tobacco is nuclearly encoded. A Su allele at this locus acts as a dominant semilethal mutation and causes reduced accumulation of chlorophyll, resulting in a yellow color in the plant. An engineered transposon tagging system, based upon the maize element Ac/Ds, was used to mutate the gene. High frequency of transposon excision from the Su locus produced variegated sectors. Plants regenerated...
Topics
- Base Sequence
- DNA Transposable Elements
- DNA, Plant
- Genetic Engineering
- Genetic Linkage
- Iron-Sulfur Proteins
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Plants, Toxic
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Nicotiana
- Zea mays