Increased synthesis of an Escherichia coli membrane protein suppresses F exclusion of bacteriophage T7.

doi:10.1006/jmbi.1999.3088

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Article

Increased synthesis of an Escherichia coli membrane protein suppresses F exclusion of bacteriophage T7.

Journal of molecular biology - 24 Sept 1999

Wang W F, Margolin W, Molineux I J

Abstract excerpt

Increased synthesis of the protein FxsA alleviates the exclusion of T7 in cells harboring the F plasmid. In contrast to wild-type or cells defective in fxsA, overexpression of fxsA+ allows T7 to form plaques at normal efficiency even though the burst size is reduced to about half that obtained on the isogenic F- strain. No defect in DNA synthesis was observed but late protein synthesis remains partially inhibited...

Topics

Amino Acid Sequence
Bacterial Proteins
Bacteriophage T7
DNA Replication
DNA, Bacterial
Escherichia coli
Escherichia coli Proteins
F Factor
Gene Expression Regulation, Bacterial
Membrane Proteins
Microscopy, Fluorescence
Molecular Sequence Data
Mutation
Nucleotides
Recombinant Fusion Proteins
Suppression, Genetic

Source

Open in PubMed

Identifiers

PMID: 10497017
DOI: 10.1006/jmbi.1999.3088

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