Article
An efficient multiplex PCR suitable for large scale typing in linkage mapping.
The Journal of veterinary medical science - 1 Jul 1999
Wei K, Wei S, Moralejo D H, Yamada T, Ogino T, Matsumoto K
Abstract excerpt
The dissection of polygenic traits is made possible with the development of microsatellite markers. Linkage study of this kind involves many markers with tens of hundreds of samples. Although typing essentially contains only two steps: PCR amplification and gel electrophoresis. Such work is still heavy when a large number of samples had to be genotyped. Multiplex PCR may reduce the work, but one has to optimize...
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