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Article

Insertional Inactivation of Treponema denticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

15 Jun 1999

Abstract excerpt

The treponemal fla operon is comprised of numerous motility-related genes; however, the initial gene of this operon, tap1, has no known function. A recently developed system to generate specific mutants in Treponema denticola was utilized to determine if Tap1 was essential for motility. T. denticola tap1 and flanking DNA were identified, cloned, and sequenced, and a suicide plasmid that contained tap1 interrupted...

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PMID: 10368149
DOI: 10.1128/jb.181.12.3743-3750.1999

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