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Article

Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis.

Applied and environmental microbiology - 1 Jun 1999

Smit E, Leeflang P, Glandorf B, van Elsas J D, Wernars K

Abstract excerpt

Like bacteria, fungi play an important role in the soil ecosystem. As only a small fraction of the fungi present in soil can be cultured, conventional microbiological techniques yield only limited information on the composition and dynamics of fungal communities in soil. DNA-based methods do not depend on the culturability of microorganisms, and therefore they offer an attractive alternative for the study of...

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Identifiers

PMID: 10347051
DOI: 10.1128/AEM.65.6.2614-2621.1999
PMCID: PMC91386

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