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Serotyping of Adenoviruses on Conjunctival Scrapings by PCR and Sequence Analysis

1 Jun 1999

Abstract excerpt

To detect and identify adenovirus (Ad), we investigated hypervariable regions (HVRs) of Ad by using a combination of PCR and direct sequencing (PCR-sequence) method. Primers for nested PCR to amplify the conserved region in the hexon protein containing HVRs were designed based on hexon gene sequences derived from GenBank. These two primer sets amplified a DNA fragment of 7 HVRs from 16 prototypes of Ad, which...

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PMID: 10325334
DOI: 10.1128/jcm.37.6.1839-1845.1999

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