Article
Conversion of dTDP-4-keto-6-deoxyglucose to free dTDP-4-keto-rhamnose by the rmIC gene products of Escherichia coli and Mycobacterium tuberculosis.
Microbiology (Reading, England) - 1 Mar 1999
Stern Richard J, Lee Tae-Yoon, Lee Tae-Jin, Yan Wenxin, Scherman Michael S, Vissa Varalakshmi D, Kim Soo-Ki, Wanner Barry L, McNeil Michael R
Abstract excerpt
dTDP-rhamnose is made from glucose-1-phosphate and dTTP by four enzymes encoded by rmIA-D. An Escherichia coli rmIC mutant was constructed and a crude enzyme extract prepared from it did not produce dTDP-4-keto-rhamnose, in contrast to a crude enzyme extract prepared from a wild-type E. coli strain where small amounts of this intermediate were found after incubation with dTDP-glucose in the absence of NADPH....
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