Article
Schizosaccharomyces pombe produces novel Gal0-2Man1-3 O-linked oligosaccharides.
Glycobiology - 1 May 1999
Gemmill T R, Trimble R B
Abstract excerpt
Schizosaccharomyces pombe whole-cell glycoproteins, previously depleted of N-linked glycans by sequential treatment with endo-ss-N-acetylglucosaminidase H and peptide-N4-asparagine amidohydrolase F, were ss-eliminated with 0.1 M NaOH/1 M NaBH4 to release the O-linked oligosaccharides. The saccharide-alditols were separated by gel-exclusion chromatography into pools from Hexitol to Hex4Hexitol in size. Analysis of...
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