Article
A Novel Trans-Complementation Assay Suggests Full Mammalian Oocyte Activation Is Coordinately Initiated by Multiple, Submembrane Sperm Components1
1 Mar 1999
Abstract excerpt
To initiate normal embryonic development, an egg must receive a signal to become activated at fertilization. We here report that the ability of demembranated sperm heads to activate is abolished after incubation over the range 20-44 degreesC and is sensitive to reducing agents. On the basis of this observation, we have developed a microinjection-based, trans-complementation assay in order to dissect the...
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