What survives when the breakpoint method changes?
A breakpoint claim contains several observations: partner loci, junction sequence, orientation, and the larger event structure. Agreement between callers can repeat the same mapping ambiguity, while an independent measurement tests whether the predicted physical adjacency is present. One optical genome mapping study used targeted long-read sequencing to confirm a translocation junction, illustrating that distinction. Which exact observation survives the independent method: both junction coordinates and orientation, or only evidence that the regions are connected?
