Back to search

Article

Cost-Effective PCR-Based Identification of T<em>unga penetrans</em> (Siphonaptera) Larvae Extracted From Soil Samples Containing PCR Inhibitor-Rich Material

2022-11-10

Abstract excerpt

Tungiasis is a neglected tropical disease caused by skin-penetrating female Tunga penetrans fleas. Although tungiasis causes severe health problems, its ecology is poorly understood and morphological descriptions of larvae are unavailable. To identify T. penetrans immature stages and sites where they develop, diagnostic PCRs are required. However, flea larvae feed on soil organic matter rich in PCR inhibitors. Her...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
fe6a2dbf-d012-5423-833d-d08235dfbef2
DOI
10.20944/preprints202211.0203.v1
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Cost-Effective PCR-Based Identification of T<em>unga penetrans</em> (Siphonaptera) Larvae Extracted From Soil Samples Containing PCR Inhibitor-Rich MaterialDOI 10.20944/preprints202211.0203.v1
Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich MaterialSemantic proximity
The complete mitochondrial genome of <i>Tunga penetrans</i> and insights into flea phylogenySemantic proximity
Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseasesSemantic proximity
Development of a protocol for soil transmitted helminths DNA extraction from feces by combining commercially available solutionsSemantic proximity
Evaluation of DNA extraction methods on individual helminth egg and larval stages for whole genome sequencingSemantic proximity
Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCRSemantic proximity
Improving eDNA filtration and purification for qPCR detection of <i>Schistosoma mansoni</i> in lakes Albert and VictoriaSemantic proximity
Development of a Molecular Snail Xenomonitoring Assay to Detect Schistosoma haematobium and Schistosoma bovis Infections in their Bulinus Snail Hosts.Semantic proximity
Tunga penetrans: molecular identification of Wolbachia endobacteria and their recognition by antibodies against proteins of endobacteria from filarial parasitesSemantic proximity
Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue.Semantic proximity
To beat or not to beat a tick: comparison of DNA extraction methods for ticks ( Ixodes scapularis )Semantic proximity
Less is More--Optimization of DNA Extraction from Canine Feces.Semantic proximity
Select a neighboring publication to make it the new centre.