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A Novel and Cost-Friendly Expression Method of Taq DNA Polymerase Using Milk Powder and Effective Utilization of the Enzyme in Diagnostic Kits

2024-05-10

Abstract excerpt

<title>Abstract</title> <p>Taq DNA polymerase has been used in PCR-based pathogen detection kits and the demand increased during the COVID-19 epidemic. In this study, it was aimed to produce recombinant Taq DNA polymerase in a low-cost and optimized setting and test its activity in various qPCR kits. Taq DNA polymerase gene was amplified by PCR and cloned into expression vectors. Recombinant protein expression wa...

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Literature Corpus work
f5479522-a23e-50f2-9d5c-ef10526ca881
DOI
10.21203/rs.3.rs-4316720/v1
Open publication

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