Article

The use of allele-specific oligonucleotide probes to characterize resistance to benomyl in field strains of Venturia inaequalis

1992-11-01

Abstract excerpt

A procedure was developed for detecting point mutations in the beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis, using the polymerase chain reaction (PCR) in combination with allele-specific oligonucleotide (ASO) analysis. PCR was used to amplify a specific 1,191-bp DNA sequence of the beta-tubulin gene in DNA extracts from axenically grown mycelium or individual apple scab lesions. The...

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Literature Corpus work: f1476c63-02c9-51c0-8422-2dc76f59b5d2
DOI: 10.1094/phyto-82-1354

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