Article

Detection of porcine circoviruses in clinical specimens using multiplex PCR in Hubei, central China

2018-08-27

Abstract excerpt

In order to detect and simultaneously discriminate PCV1, PCV2 and PCV3, a multiplex PCR assay was developed and used to detect clinical samples in this study. Each of target genes of PCV1, PCV2 and PCV3 was amplified using the designed primers, while no other porcine viruses genes were detected. The limit of detection of the assay was 10 copies/μL of PCV1, PCV2 and PCV3. The tissue samples from eight pig farms wer...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: eb052954-e8d5-56fe-b3d0-1aa3137ff074
DOI: 10.7287/peerj.preprints.27145v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Detection of porcine circoviruses in clinical specimens using multiplex PCR in Hubei, central ChinaDOI 10.7287/peerj.preprints.27145v1Read

Development of a multiplex PCR to detect and discriminate porcine circoviruses in clinical specimensSemantic proximity Read

Detection and genetic characteristics of porcine circovirus type 2 and 3 in Henan province of China.Semantic proximity Read

Simultaneous detection and genetic characterization of porcine circovirus 2 and 4 in Henan province of China.Semantic proximity Read

Co-infection status of porcine circoviruses (PCV2 and PCV3) and porcine epidemic diarrhea virus (PEDV) in pigs with watery diarrhea in Henan province, central ChinaSemantic proximity Read

Epidemiological investigation and analysis of the infection of porcine circovirus in Xinjiang.Semantic proximity Read

Prevalence and genetic analysis of porcine circovirus type 2 (PCV2) and type 3 (PCV3) between 2018 and 2020 in central China.Semantic proximity Read

Detection and phylogenetic analysis of porcine circovirus type 3 in central China.Semantic proximity Read

Co-infection status of PCV2 and PRRSV with other pathogens in Porcine Circovirus Associated Disease and Porcine Respiratory Disease ComplexSemantic proximity Read

The Prevalence and Genetic Diversity of Porcine Circoviruses (PCVs) during 2017–2023 in Guangdong Province, ChinaSemantic proximity Read

Molecular detection and phylogenetic analysis of porcine circovirus type 3 in 21 Provinces of China during 2015–2017Semantic proximity Read

Prevalence and phylogenetic analysis of porcine circovirus type 2 (PCV2) and type 3 (PCV3) in the Southwest of China during 2020–2022Semantic proximity Read

Phylogenetic Analysis and Serological Investigation of Porcine Circovirus Indicates Frequent Infection with Various Subtypes.Semantic proximity Read