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A duplex-PCR bioassay to detect a Trichoderma virens biocontrol isolate in non-sterile soil

2004-12-01

Abstract excerpt

This paper describes the identification and utilisation of a sequence-characterised amplified region (SCAR) marker specific for the Trichoderma virens biocontrol isolate GV4. The marker was developed from a RAPD-PCR amplification product unique to isolate GV4. When used as a hybridisation probe in Southern blot analysis, it hybridised to the DNA of the species T. virens alone and not to that of other Trichoderma s...

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Literature Corpus work
e84dc9df-5cd0-50bb-a67a-bd4ded21d8e7
DOI
10.1016/j.soilbio.2004.03.012
Open publication

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A duplex-PCR bioassay to detect a Trichoderma virens biocontrol isolate in non-sterile soilDOI 10.1016/j.soilbio.2004.03.012
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