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Detection and Identification of Brenneria nigrifluens, the Causal Agent of the Shallow Bark Canker of Walnut by, PCR Amplification

2008-08-01

Abstract excerpt

A 1 kb DNA band from strains of Brenneria nigrifluens, as shown by amplification of their genomic DNA by polymerase chain reaction (PCR) using minisatellite primer designed on the minisatellite sequence of the M13 phage, was isolated, cloned and sequenced. Specific oligonucleotides (F1-C3) were selected into this 1 kb DNA sequence and used in a PCR assay to detect and identify strains of B. nigrifluens. Several st...

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Identifiers and source

Literature Corpus work
e6a76a7e-72c2-50b9-a69a-f533f8618712
DOI
10.1111/j.1439-0434.2007.01393.x
Open publication

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