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Establishment and validation of a duplex, real-time PCR assay for detection of African swine fever virus

2024-01-30

Abstract excerpt

The outbreak of African swine fever (ASF) in China in 2018, caused a huge economic loss on the pig industry. To accurately diagnose clinical infection with ASF virus (ASFV), we developed a duplex TaqMan-probe-based real-time PCR for the simultaneous detection of two discontinuous genes in the virus genome to avoid false-negative in the clinical diagnosis. Two sets of ASFV-gene specific primers, along with two TaqM...

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Literature Corpus work
db7a60a6-78b6-5ced-a6de-78314f33b3ae
DOI
10.22541/au.170664510.04182787/v1
Open publication

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Establishment and validation of a duplex, real-time PCR assay for detection of African swine fever virusDOI 10.22541/au.170664510.04182787/v1
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