Article

Integration of RNA-seq and RNAi provides a novel insight into the effect of pvdE gene to the pathogenic of Pseudomonas plecoglossicida and on the immune responses of orange-spotted grouper (Epinephelus coioides)

2021-12-01

Abstract excerpt

Pseudomonas plecoglossicida is an important pathogen of maricultured fish in China and caused great loss. In this study, the mRNA level of pvdE gene of wild-type strain of P. plecoglossicida (NZBD9) was significantly decreased by 5 shRNAs and the mutant with the best silencing efficiency (89.4%) were chosen for subsequent research. Injection of pvdE-RNAi strain at a dose of 10⁵ cfu/fish did not cause death in oran...

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Literature Corpus work: d0a53eba-a21b-5a47-a1a1-b2b7113eb53e
DOI: 10.1016/j.aquaculture.2020.735695

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