Development and validation of cost-effective SYBR Green-based RT-qPCR and its evaluation in a sample pooling strategy for detecting SARS-CoV-2 infection in the Indonesian setting

Abstract excerpt

Coronavirus disease 2019 or COVID-19 is caused by SARS-CoV-2 infection and it was first recorded in Wuhan, China, in December 2019. The gold standard method for detecting SARS-CoV-2 is TaqMan probe-based real-time polymerase chain reaction (RT-qPCR). Since this technique is expensive due to reliance on the use of fluorogenic probes, a cost-effective diagnostic test as an alternative approach for RT-qPCR diagnosis...

Topics

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• Advanced biosensing and bioanalysis techniques
• Biosensors and Analytical Detection
• Molecular Biology Techniques and Applications
• Respiratory viral infections research
• SARS-CoV-2 and COVID-19 Research
• SARS-CoV-2 detection and testing

Identifiers and source

Literature Corpus work

d077b598-cf62-5e8a-90b2-85a24005ae74

DOI

10.21203/rs.3.rs-2867074/v1