Article

Smartphone-integrated RPA-CRISPR-Cas12a Detection System with Microneedle Sampling for Point-of-Care Diagnosis of Potato Late Blight in Early Stage

2025-06-18

Abstract excerpt

Potato late blight, caused by the oomycete pathogen Phytophthora infestans , is one of the most devastating diseases affecting potato crops in the history. Although conventional detection methods of plant diseases such as PCR and LAMP are highly sensitive and specific, they rely on bulky and expensive laboratory equipment and involve complex operations, making them impracticable for point-of care diagnosis in the...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: cd8eaa3d-e0e8-5e20-9e78-32ce502bfb9a
DOI: 10.1101/2025.06.17.660259

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Smartphone-integrated RPA-CRISPR-Cas12a Detection System with Microneedle Sampling for Point-of-Care Diagnosis of Potato Late Blight in Early StageDOI 10.1101/2025.06.17.660259Read

Ultrasensitive, Low-Input Detection of Avocado Sunblotch Viroid via RPA-CRISPR and Nanopore-Array Single-Bead Fluorescence ReadoutSemantic proximity Read

Precise in-field molecular diagnostics of crop diseases by smartphone-based mutation-resolved pathogenic RNA analysis.Semantic proximity Read

Advancements and applications of loop‐mediated isothermal amplification ( LAMP ) technology in the detection of viral diseases in fruit cropsSemantic proximity Read

Development and Validation of Genome-Informed and Multigene-based qPCR and LAMP Assays for Accurate Detection of <i>Dickeya solani</i> : A Critical Quarantine Pathogen Threatening Potato IndustrySemantic proximity Read

Rapid and sensitive on-site genetic diagnostics of pest fruit flies using CRISPR-Cas12aSemantic proximity Read

Do CRISPR-based disease diagnosis methods qualify as point-of-care diagnostics for plant diseases?Semantic proximity Read

A Review of Available eDNA Sampling Methods to Detect Plant Pathogenic Organisms in Horticultural SettingsSemantic proximity Read

CRISPR-based diagnostics detects invasive insect pestsSemantic proximity Read

Enhanced Diagnosis of Tomato Brown Rugose Fruit Virus (TBRFV) Infections Through CRISPR-Cas12 and CRISPR-Cas9 TechnologiesSemantic proximity Read

PAM-free loop-mediated isothermal amplification coupled with CRISPR/Cas12a cleavage (Cas-PfLAMP) for rapid detection of rice pathogensSemantic proximity Read

Spraying of dsRNA Molecules Derived from Phytophthora Infestans, as a Plant Protection Strategies for the Management of Potato Late BlightSemantic proximity Read

Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in riceSemantic proximity Read