Article

Transformation of Bipolaris sorokiniana with the GUS gene and use for studying fungal colonization of barley roots

1993-12-01

Abstract excerpt

Bipolaris sorokiniana, a fungal pathogen of cereals, was transformed with the GUS (beta-glucuronidase) gene, using a plasmid (pGUS5) containing GUS A with the constitutive promotor GPD-1 and a hygromycin-resistance gene (hph) under the control of the same promotor. Hygromycin resistance was used as the selectable marker. Colonies growing on medium with hygromycin were obtained at a frequency of 6.5 X 10(-7) per vi...

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Literature Corpus work: bff39a9c-dfb8-57a2-aef0-fa13de2fa45e
DOI: 10.1094/phyto-83-1484

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